Microbiology An Introduction Test Bank

Correct Answer: B

Rationale: The correct answer is B: Actinomycosis. Actinomyces is a gram-positive bacterium that forms sulfur granules (druses) with gram-positive coloring in the center and filamentous structures resembling cones with gram-negative coloring. This morphology is characteristic of Actinomyces species. Fusobacteriosis (choice A) typically presents with gram-negative rods. Staphylococcal osteomyelitis (choice C) is caused by gram-positive staphylococci, not Actinomyces. Anaerobic infections (choice D) can be caused by various bacteria, but the specific morphology described in the question matches Actinomyces, making choice B the correct answer.

Correct Answer: C

Rationale: The correct answer is C: cell wall composition. Gram staining differentiates bacteria based on the structure of their cell walls. It classifies bacteria into Gram-positive (thick peptidoglycan layer) and Gram-negative (thin peptidoglycan layer). This differentiation is crucial for identifying bacterial species and guiding treatment choices. Size and shape (choice A) are not specific to Gram staining. Presence of flagella (choice B) is related to bacterial motility, not Gram staining. Genetic material (choice D) is not directly assessed by Gram staining. Overall, the unique composition of the cell wall is the key factor in distinguishing bacteria through Gram staining.

Correct Answer: D

Rationale: Interferon delta is not a known type of interferon, making choice D incorrect. Interferon beta is indeed produced by fibroblasts (choice A), interferon alpha by leukocytes (choice B), and interferon gamma by lymphocytes (choice C). Each type of interferon is produced by specific cells in the immune system, contributing to the body's defense against viruses and other pathogens.

Correct Answer: A

Rationale: The correct answer is A: Spores. The Ozheshko method is used to stain spores, which are resistant structures produced by certain bacterial species. By studying the smear of streptobacillus preparation under oil immersion, one can observe the presence of spores as distinct, oval bodies within the bacteria. Spores are important for bacterial survival in harsh conditions. Summary: B: Capsules are not typically stained using the Ozheshko method. Capsules are usually visualized using negative staining techniques. C: Flagella are not stained by the Ozheshko method. Flagella are visualized using special staining techniques like the flagella stain. D: Inclusions are not specifically stained by the Ozheshko method. Inclusions are often observed using techniques that highlight cellular structures or components.

Correct Answer: B

Rationale: The correct answer is B: Actinomycosis. Actinomyces is a gram-positive bacterium that forms sulfur granules (druses) with gram-positive coloring in the center and filamentous structures resembling cones with gram-negative coloring. This morphology is characteristic of Actinomyces species. Fusobacteriosis (choice A) typically presents with gram-negative rods. Staphylococcal osteomyelitis (choice C) is caused by gram-positive staphylococci, not Actinomyces. Anaerobic infections (choice D) can be caused by various bacteria, but the specific morphology described in the question matches Actinomyces, making choice B the correct answer.