medical microbiology test bank

Correct Answer: C

Rationale: The correct answer is C: crateriform. In a gelatin stab, a saucer-shaped liquefaction indicates a depression or crater-like shape. This term accurately describes the appearance of the liquefaction. A: napiform means turnip-shaped, which is not relevant to the shape in a gelatin stab. B: saccate means pouch-like, which does not match the saucer-shaped description. D: stratiform refers to something in layers, which is not applicable to the shape of the liquefaction. Therefore, C is the most appropriate term to describe the saucer-shaped liquefaction in a gelatin stab.

Correct Answer: A

Rationale: The correct answer is A: Tetanus. Tetanus is caused by the bacterium Clostridium tetani, which produces a neurotoxin leading to muscle stiffness, including trismus (restricted mouth opening). The patient's history of a stab wound increases the likelihood of tetanus infection due to the spores entering the body through the wound. Incorrect Choices: B: Brucellosis does not typically present with trismus and is more commonly associated with fever, joint pain, and fatigue. C: Whooping cough presents with severe coughing fits but does not cause trismus. D: Wound anaerobic infection may cause localized symptoms at the wound site but is less likely to cause trismus compared to tetanus.

Correct Answer: D

Rationale: The correct answer is D: Protoplast. Lipopolysaccharides of Gram-negative bacteria consist of three main components: O-specific polysaccharide chain, R-specific polysaccharide chain, and Lipid A. Protoplast is not a component of lipopolysaccharides. O-specific polysaccharide chain is responsible for serotype specificity, R-specific polysaccharide chain varies among strains, and Lipid A is the endotoxin component. Protoplast refers to a bacterial cell devoid of a cell wall, which is not a component of lipopolysaccharides.

Correct Answer: D

Rationale: The correct answer is D: decimal reduction time. This term represents the time required at a specific temperature to kill 90% of a bacterial population. It is a key parameter in microbiology to determine the effectiveness of a given heat treatment. Generation time (A) refers to the time it takes for a population to double in size. Thermal death time (B) is the shortest time needed to kill all organisms at a specific temperature. Sterilization time (C) is a general term and does not specifically refer to the time required to kill 90% of the population.

Correct Answer: B

Rationale: The correct answer is B: autoclaving. Autoclaving is a process that uses high pressure and steam to achieve temperatures above the boiling point of water, typically around 121-134�C. This high heat and pressure are effective in destroying spore forms of microorganisms, which are highly resistant structures that can withstand boiling temperatures. Autoclaving ensures the complete destruction of spores, making it an effective method for sterilization. Rationale: A: Pasteurization uses lower heat compared to autoclaving and is not sufficient to destroy spore forms. C: Freezing does not effectively destroy spore forms, as most microorganisms can survive freezing temperatures. D: Boiling, while effective for many microorganisms, may not be sufficient to destroy spore forms due to their high resistance, unlike autoclaving.