Microbiology Test Bank

Correct Answer: A

Rationale: The correct answer is A: Peroxidase and catalase. Peroxidase and catalase are enzymes that break down hydrogen peroxide into water and oxygen. Peroxidase helps in breaking down low levels of hydrogen peroxide, while catalase is crucial for breaking down high levels of hydrogen peroxide efficiently. This process is important for organisms to prevent damage from reactive oxygen species. Choices B, C, and D are incorrect: B: Oxygenase and hydroxylase are enzymes involved in different biochemical reactions and do not break down hydrogen peroxide. C: Cytochrome oxidase and cytochrome B5 are involved in electron transport chain reactions and not in breaking down hydrogen peroxide. D: Oxygenase and catalase are not correct as oxygenase is not involved in hydrogen peroxide breakdown, only catalase is.

Correct Answer: D

Rationale: The correct answer is D: Fifth disease. Rubella virus is associated with Fifth disease, also known as erythema infectiosum. It is caused by parvovirus B19, not rubella virus. Mumps (choice A) is caused by the mumps virus. Roseola (choice B) is caused by human herpesvirus 6 or 7. Rubeola (choice C) is the scientific name for measles, caused by the measles virus. Therefore, the correct answer is Fifth disease as it is the disease associated with rubella virus.

Correct Answer: A

Rationale: The correct answer is A: Candida fungi. Candida fungi are known to cause oral thrush, characterized by whitish spots on the oral mucosa. The presence of gram-positive oval budding cells in the smear preparations is indicative of Candida species. Staphylococci (choice B) are gram-positive cocci, not oval budding cells. Sarcinae (choice C) are gram-positive bacteria that form cuboidal packets, not oval budding cells. Actinomycetes (choice D) are filamentous gram-positive bacteria, not oval budding cells like Candida fungi.

Correct Answer: D

Rationale: The correct answer is D, Klebsiella pneumoniae. Klebsiella pneumoniae is a fermentative gram-negative rod, which means it can ferment sugars. In contrast, Pseudomonas aeruginosa, Acinetobacter baumannii, and Stenotrophomonas maltophilia are non-fermentative gram-negative rods. These organisms do not ferment sugars for energy production. Therefore, Klebsiella pneumoniae is the only fermentative organism among the choices, making it the correct answer.

Correct Answer: A

Rationale: The correct answer is A: Leffler's method. In this staining method, gentian violet solution is used as the primary stain, followed by Lugol's solution as a mordant, 960 spiritus as a decolorizer, and water fuchsin as a counterstain. Gentian violet stains the organisms purple, Lugol's solution helps in fixing the dye, 960 spiritus decolorizes non-specifically, and water fuchsin acts as a counterstain to help visualize the bacteria. Leffler's method is specifically used for staining sputum smears in suspected cases of croupous pneumonia. Summary: B: Gram's staining uses crystal violet, iodine, alcohol, and safranin. It is mainly used for distinguishing between Gram-positive and Gram-negative bacteria. C: Ziehl-Neelsen's staining is used for acid-fast bacteria like Mycobacterium tuberculosis using carbol fuchsin and acid-alcohol