OpenStax Microbiology Test Bank

Correct Answer: D

Rationale: The correct answer is D: Loefler. This staining method is used for identifying Corynebacterium diphtheriae, the causative agent of diphtheria. It involves using methylene blue and basic fuchsin to stain the bacteria. The yellow bacilli with blue grains at their ends indicate the characteristic appearance of C. diphtheriae when stained using the Loefler method. Choice A: Ziehl-Nielsen is used for acid-fast bacteria like Mycobacterium tuberculosis. Choice B: Kozlovsky is not a standard staining method in microbiology. Choice C: Romanovsky is used for blood smears to identify malaria parasites.

Correct Answer: D

Rationale: The correct answer is D. Sepsis is a systemic response to infection resulting in organ dysfunction. A: Sepsis can be caused by bacterial infection, but it is not solely defined by the presence of bacteria in the blood. B: Bacteremia is the presence of bacteria in the blood, which can be a component of sepsis, but sepsis involves a broader systemic response beyond just bacteremia. C: Sepsis can lead to the release of toxins and organ failure, but sepsis itself is not defined solely by the massive proliferation of toxins. Therefore, the correct answer is D, as sepsis can involve the presence of bacteria, bacteremia, systemic manifestations, and organ failure.

Correct Answer: A

Rationale: The correct answer is A: Norwalk Virus. The Norwalk Virus belongs to the family Caliciviridae because it has characteristic features such as a nonenveloped, single-stranded RNA genome. The other options, Yellow fever virus, Rubella virus, and CMV, belong to different virus families and have distinct characteristics that differentiate them from Caliciviridae. Yellow fever virus is a Flavivirus, Rubella virus belongs to Togaviridae, and CMV is a member of the Herpesviridae family. Therefore, based on the genomic and structural characteristics of the Norwalk Virus, it is the correct choice for belonging to the Caliciviridae family.

Correct Answer: A

Rationale: The correct method of laboratory diagnostics for suspected malaria is blood examination. Malaria is diagnosed by detecting the presence of the Plasmodium parasite in the patient's blood. This can be done through microscopy to identify the parasite in a blood smear or through rapid diagnostic tests (RDTs) that detect specific antigens produced by the parasite. Blood examination allows for the direct visualization of the parasite, making it the most accurate method for diagnosing malaria. Immunological tests, stool examination, and urine examination are not suitable for diagnosing malaria as the parasite primarily resides in the red blood cells. Stool and urine examinations are more commonly used for diagnosing gastrointestinal or urinary tract infections, respectively.

Correct Answer: D

Rationale: The correct answer is D because the rubella virus has icosahedral symmetry, meaning it has a spherical shape with 20 equilateral triangular faces. This symmetry is common in many viruses and allows for efficient packaging of genetic material. Choice A is incorrect because the rubella virus does have glycoprotein spikes on its surface. These spikes help the virus attach to host cells. Choice B is incorrect because the rubella virus does have an envelope derived from the host cell membrane. This envelope plays a role in viral entry into host cells. Choice C is incorrect because the rubella virus is an ssRNA virus, not ssDNA. Its genetic material is single-stranded RNA, not DNA. In summary, the rubella virus has icosahedral symmetry and possesses glycoprotein spikes, an envelope, and ssRNA genetic material, making choices A, B, and C incorrect.