microbiology chapter 10 test bank

Correct Answer: A

Rationale: Step-by-step rationale for why the correct answer is A (Cholera): 1. Gram-negative curved mobile monotrichous bacilli: Vibrio cholerae, the causative agent of cholera, fits this description. 2. Do not produce spores or capsules: Vibrio cholerae does not produce spores or capsules. 3. Require alkaline reaction (pH 8.5-9.5): Vibrio cholerae thrives in alkaline environments. 4. Enteric infection: Cholera is a classic example of an enteric infection transmitted through contaminated food or water. Summary of why other choices are incorrect: B: Shigellosis is caused by Shigella species, which are gram-negative, but they are not curved bacilli and do not require an alkaline pH. C: Typhoid fever is caused by Salmonella Typhi, which are gram-negative but are not curved bacilli and do not require an alkaline pH.

Correct Answer: A

Rationale: Step 1: Ziehl-Neelsen staining is a type of acid-fast staining used to detect acid-resistant bacteria like Mycobacterium species. Step 2: Acid-resistant bacteria have a high lipid content in their cell walls, making them resist decolorization with acid-alcohol. Step 3: Mycobacterium species, such as M. tuberculosis and M. leprae, are examples of acid-resistant bacteria. Step 4: Spores are not typically stained using Ziehl-Neelsen staining, making choice B incorrect. Step 5: Metachromatic bodies are stained using specific techniques like Albert's stain, not Ziehl-Neelsen staining, making choice C incorrect. Summary: Ziehl-Neelsen staining is specifically used for acid-resistant bacteria detection, making choice A the correct answer. Choices B and C are incorrect as they do not align with the purpose of Ziehl-Neelsen staining. Choice D is incorrect as Ziehl

Correct Answer: C

Rationale: The correct answer is C because Gram staining after culture on Loeffler's medium or tellurite-containing medium will show the characteristic Chinese letter arrangement of Corynebacterium diphtheriae. This arrangement consists of metachromatic granules that stain more deeply than the rest of the cell. This specific staining pattern is a key diagnostic feature of C. diphtheriae in microbiological diagnosis of diphtheria. Choice A is incorrect because simply identifying the isolate as C. diphtheriae is not sufficient for diagnosis. Choice B is incorrect as C. diphtheriae can be stained via Gram staining despite its small cell size. Choice D is incorrect as option C is indeed correct for microbiological diagnosis of diphtheria.

Correct Answer: A

Rationale: The correct answer is A: ocular lens. The ocular lens is the lens closest to the eye in a compound light microscope. This lens is responsible for magnifying the image produced by the objective lens. It typically has a magnification power of 10x. The objective lens, choice B, is positioned closer to the specimen and provides the primary magnification of the image. The condenser, choice C, is located beneath the stage and helps focus light onto the specimen. The stage, choice D, is where the specimen is placed for observation. Therefore, the ocular lens is the correct choice as it is the lens closest to the eye and responsible for further magnifying the image.

Correct Answer: B

Rationale: The correct answer is B: Hantavirus. Hantaviruses are not arthropod-borne, unlike the other choices. Hantaviruses are transmitted to humans primarily through contact with rodent urine, feces, or saliva. Phlebovirus (A), Nairovirus (C), and Uukuvirus (D) are all bunyaviruses that are transmitted by arthropod vectors such as mosquitoes or ticks. Therefore, Hantavirus is the correct answer as it is not transmitted by arthropods.