microbiology chapter 14 test bank

Correct Answer: A

Rationale: The correct answer is A: Cytopathic effect. This is because polioviruses cause visible changes in infected cells, leading to cell damage and death, known as cytopathic effect. This is a key characteristic of viral infection. Choice B (Cytotoxic effect) is incorrect as it refers to the toxicity of a substance to cells, not the specific effect of polioviruses. Choice C (Cytolytic effect) is incorrect as it specifically refers to cell lysis, which is not the primary mechanism of poliovirus infection. Choice D (They Don't Change The Cell Cultures) is incorrect because polioviruses do indeed cause changes in cell cultures due to their cytopathic effect.

Correct Answer: C

Rationale: The correct answer is C: Papillomaviruses. Papillomaviruses are primarily transmitted through sexual contact, as they infect the skin and mucous membranes of the genital area. This transmission route is well-documented through research and epidemiological studies. Arenaviruses (A) are primarily transmitted through contact with infected rodents, not sexual contact. Togaviruses (B) are mainly transmitted by mosquitoes and ticks. Polyomaviruses (D) are typically spread through respiratory secretions or contaminated urine, not through sexual contact. Therefore, the most appropriate choice for viruses transmitted mainly through sexual contact is Papillomaviruses (C).

Correct Answer: C

Rationale: The correct answer is C: Direct flaming. Direct flaming is the simplest heat-related method to sterilize metal because it involves passing the metal through a flame to kill microorganisms. The high temperature of the flame effectively destroys any bacteria or spores present on the metal surface. Incineration (A) is not practical for sterilizing metal as it involves burning at extremely high temperatures. Autoclaving (B) uses steam under pressure to sterilize, which is effective but more complex than direct flaming. Indirect heating (D) is not an effective method for sterilizing metal as it does not directly expose the metal to high temperatures required for sterilization.

Correct Answer: A

Rationale: The correct answer is A: Ziehl-Neelsen staining method. This method is used to detect acid-fast bacteria like Mycobacterium tuberculosis. Step-by-step rationale: 1. Acid-fast bacteria resist decolorization with acid-alcohol. 2. The red bacilli seen in the smear indicate acid-fast bacteria. 3. Ziehl-Neelsen stain uses carbol fuchsin to stain acid-fast bacteria red. 4. The blue background is likely the counterstain methylene blue. Incorrect choices: B: Gram stain is for categorizing bacteria into Gram-positive or Gram-negative, not specific for acid-fast bacteria. C: Giemsa stain is used for blood films and detecting parasites, not acid-fast bacteria. D: Neisser stain is not a standard microbiological staining method.

Correct Answer: A

Rationale: The correct answer is A: Hantavirus. Hantavirus is considered a newly emerging disease because it was first identified in the late 20th century. It is transmitted to humans through rodents and can lead to severe respiratory illness. Malaria, Tuberculosis, and Chlamydia are not considered newly emerging diseases as they have been known for centuries and continue to be prevalent worldwide. Therefore, based on the timeline of discovery and emergence, Hantavirus is the most recent infectious disease among the options provided.