microbiology an evolving science test bank

Correct Answer: A

Rationale: The correct answer is A: Two-layer. In gram-positive bacteria, the cell envelope consists of two layers: the thick peptidoglycan layer and the cytoplasmic membrane. The peptidoglycan layer is much thicker compared to gram-negative bacteria, providing structural support and protection. The cytoplasmic membrane is located beneath the peptidoglycan layer and serves as a barrier between the cytoplasm and the external environment. The other choices are incorrect because gram-positive bacteria do have a cell envelope, it is composed of two layers, not three layers, and it is not just a single layer. Choice D is incorrect as it states that there is no cell envelope in gram-positive bacteria, which is not true.

Correct Answer: C

Rationale: The correct answer is C: Protection against harsh conditions. Bacterial endospores are dormant structures produced by certain bacteria as a survival strategy under unfavorable conditions. They protect the bacterial DNA and other essential components from damage caused by heat, chemicals, desiccation, and radiation. Endospores do not directly participate in energy production (A), DNA replication (B), or protein synthesis (D). These functions are carried out by the active, vegetative form of the bacterial cell.

Correct Answer: A

Rationale: The correct answer is A: ethylene oxide. Ethylene oxide is a toxic, odorless gas commonly used as a sterilizing agent in healthcare settings due to its ability to effectively kill bacteria, viruses, and fungi. It is a highly reactive compound that can penetrate porous materials, making it an ideal choice for sterilization purposes. Incorrect choices: B: Carbon dioxide is not used as a sterilizing agent and is not toxic. C: Carbon monoxide is not commonly used for sterilization and has a distinct odor. D: Propane is not used for sterilization in healthcare settings and is not odorless.

Correct Answer: A

Rationale: The correct answer is A: Gram staining method. This method involves staining with gentian violet, followed by Lugol's solution, 96% alcohol decolorization, and counterstaining with safranin or water-magenta. This staining sequence allows for the differentiation of bacteria based on their cell wall composition. In the case of lobar pneumonia, the causative agent is typically a bacterium with a cell wall that retains the crystal violet stain after decolorization with alcohol, indicating a Gram-positive bacterium. Summary of Incorrect Choices: B: Ziehl-Nielsen staining is used for acid-fast bacteria like Mycobacterium tuberculosis, not for lobar pneumonia. C: Romanovsky staining is primarily used in the identification of blood cells and blood parasites, not for bacteria. D: Neisser staining is not a commonly used staining method in microbiology for bacterial identification.

Correct Answer: B

Rationale: The correct answer is B because Gram-negative bacteria have an outer membrane with lipopolysaccharides, which is a key characteristic used in Gram staining. This outer membrane is responsible for the pink/red color seen when stained with Gram stain. Choice A is incorrect because Gram-negative bacteria have a thin peptidoglycan layer. Choice C is incorrect because Gram-negative bacteria stain pink, not purple. Choice D is incorrect because all bacteria have a cell wall, but Gram-negative bacteria have a different structure compared to Gram-positive bacteria.