OpenStax Microbiology Test Bank

Correct Answer: A

Rationale: The correct answer is A: 4 and 250. Coli index is calculated by dividing the number of red colonies by the volume of water analyzed, which gives 2 red colonies / 500 ml = 4. Coli titer is the reciprocal of the coli index, so it is 1 / 4 = 250. This indicates the concentration of coliform bacteria in the water sample. Choices B, C, and D are incorrect as they do not follow the correct calculation steps for determining the coli index and coli titer. B has the correct coli index but incorrect coli titer. C and D have the values reversed, leading to incorrect results.

Correct Answer: A

Rationale: The correct answer is A: Treponema. Treponema pallidum is a spirochete bacterium that appears as long, thin, and spiral-shaped microorganisms with 8-12 regular coils under the microscope. This characteristic morphology is specific to Treponema species, making it the correct choice. Borrelia, Leptospira, and Vibrios have different shapes and arrangements under the microscope, making them incorrect choices in this scenario.

Correct Answer: D

Rationale: The correct answer is D because viruses have the ability to infect all organisms, including plants, bacteria, animals, and even fungi. Viruses are non-living entities that require a host cell to replicate and spread. They can infect a wide range of organisms by attaching to specific receptors on the host cell's surface. Therefore, the correct answer is D as viruses have the capability to infect all living organisms. Choices A, B, and C are incorrect because viruses can infect not only plants, bacteria, and animals but also other organisms, making choice D the most comprehensive and accurate option.

Correct Answer: A

Rationale: The correct answer is A: Peptidoglycan. Bacterial cell walls are composed of peptidoglycan, a unique macromolecule consisting of sugars and amino acids. This structure provides rigidity and protection to the cell. Lipopolysaccharides are found in the outer membrane of Gram-negative bacteria, not in the cell wall. Phospholipids are the main components of cell membranes, not cell walls. Chitin is a structural polysaccharide found in the cell walls of fungi and some algae, not bacteria.

Correct Answer: B

Rationale: The Ziehl-Neelsen stain is specifically designed to detect acid-fast bacteria like Mycobacterium tuberculosis. It utilizes a carbol fuchsin dye that binds to the high lipid content in the cell wall of acid-fast bacteria, making them appear red under a microscope. This staining method is crucial for the diagnosis of tuberculosis and leprosy. Gram-positive bacteria (Choice A) are not detected by this stain since they have a different cell wall structure. Capsulated bacteria (Choice C) and fungal spores (Choice D) also have distinct characteristics that are not targeted by the Ziehl-Neelsen stain.