microbiology an evolving science test bank

Correct Answer: A

Rationale: The correct answer is A: Clostridium perfringens. Gas gangrene is caused by Clostridium perfringens, a Gram-positive rod bacterium that produces spores. This bacterium is anaerobic and commonly found in soil, where it forms spores that can survive harsh conditions. Clostridium tetani causes tetanus, not gas gangrene. Bacillus anthracis causes anthrax, not gas gangrene. Clostridium botulinum causes botulism, not gas gangrene. Therefore, based on the characteristics of the microorganism described in the question, Clostridium perfringens is the correct identification.

Correct Answer: A

Rationale: The correct answer is A: Gram staining method. This method involves staining with gentian violet, followed by Lugol's solution, 96% alcohol decolorization, and counterstaining with safranin or water-magenta. This staining sequence allows for the differentiation of bacteria based on their cell wall composition. In the case of lobar pneumonia, the causative agent is typically a bacterium with a cell wall that retains the crystal violet stain after decolorization with alcohol, indicating a Gram-positive bacterium. Summary of Incorrect Choices: B: Ziehl-Nielsen staining is used for acid-fast bacteria like Mycobacterium tuberculosis, not for lobar pneumonia. C: Romanovsky staining is primarily used in the identification of blood cells and blood parasites, not for bacteria. D: Neisser staining is not a commonly used staining method in microbiology for bacterial identification.

Correct Answer: A

Rationale: The correct answer is A: C. botulinum. The description of gram-positive bacilli with subterminal staining defect and tennis racket appearance is characteristic of Clostridium botulinum. The subterminal staining defect indicates the presence of spores, a feature of Clostridium species. The tennis racket appearance refers to the characteristic shape of C. botulinum spores. Choice B, P. vulgaris, is a gram-negative bacterium and doesn't match the description. Choice C, E. coli, is a gram-negative bacillus and doesn't exhibit the subterminal staining defect described. Choice D, S. aureus, is a gram-positive cocci and does not resemble the tennis racket appearance described.

Correct Answer: D

Rationale: The correct answer is D: Q-fever. Q-fever is typically an infection that affects the lower respiratory tract, causing symptoms like pneumonia. Haemophilus influenzae pharyngitis (choice A) is an upper respiratory tract infection. Ozeana (choice B) and diphtherial croup (choice C) are not specific infections related to the lower respiratory tract. Q-fever stands out as the correct choice due to its association with lower respiratory tract infections.

Correct Answer: C

Rationale: The correct answer is C: E + S = P. This notation represents the interaction between an enzyme (E) and a substrate (S) resulting in a product (P). Enzymes facilitate chemical reactions by binding to substrates and converting them into products. Choice A is incorrect because it does not include the enzyme in the reaction. Choice B is incorrect as it involves two substrates binding to the enzyme, not a substrate converting to a product. Choice D is incorrect as it shows two substrates reacting to form a product without involvement of an enzyme. Thus, choice C accurately represents the process of enzymatic reactions involving enzymes, substrates, and products.