free microbiology test bank questions pdf

Correct Answer: A

Rationale: The correct answer is A: Enteropathogenic E. Coli (EPEC). EPEC bacteria adhere to the intestine wall using a protein called intimin, leading to microvilli damage. This attachment disrupts the normal absorption function of the intestine. Summary of why other choices are incorrect: B: Enterohemorrhagic E. Coli (EHEC) primarily causes bloody diarrhea and does not specifically target microvilli. C: Enterotoxigenic E. Coli (ETEC) produces toxins that lead to watery diarrhea but does not directly target microvilli. D: Enteroinvasive E. Coli (EIEC) invades and replicates within intestinal cells, causing inflammatory diarrhea, but does not specifically damage microvilli.

Correct Answer: A

Rationale: The correct serological test for diagnosing toxoplasmosis is the Complement Binding Assay (A) because it detects antibodies specific to the Toxoplasma gondii parasite. This test is highly sensitive and specific for toxoplasmosis. Choice B, Precipitation test, is not used for toxoplasmosis diagnosis but rather for other diseases like syphilis. Choice C, Neutralization test, is not specific for toxoplasmosis and is used for other types of infections. Choice D, Widal's test, is used for diagnosing typhoid fever and is not relevant for toxoplasmosis. In summary, the correct answer (A) is specific to toxoplasmosis, while the other choices are used for different diseases and are not suitable for diagnosing toxoplasmosis.

Correct Answer: D

Rationale: The correct answer is D. Isoniazid inhibits pyridoxal phosphate synthesis, which is the active form of vitamin B6. Pyridoxal phosphate is essential for various enzymatic reactions, including neurotransmitter synthesis and myelin formation. Therefore, isoniazid-induced pyridoxal phosphate deficiency can lead to neurological symptoms like polyneuritis, paresthesia, memory disorders, and convulsions. A: Disruption of cell membrane synthesis - This is not the mechanism of isoniazid side-effects. B: Inhibition of protein synthesis - Isoniazid does not directly inhibit protein synthesis. C: Inhibition of RNA-synthesis - Isoniazid does not inhibit RNA synthesis; it affects vitamin B6 metabolism.

Correct Answer: A

Rationale: Ribosomes are responsible for protein synthesis, where they translate the genetic information from mRNA into specific amino acid sequences to form proteins. This process occurs in both prokaryotic and eukaryotic cells. Ribosomes do not participate in the breakdown of substances, which is mainly carried out by lysosomes or proteasomes. Oxidative phosphorylation involves the generation of ATP in mitochondria, not ribosomes. Ribosomes do not inactivate antibiotics; this is typically done by enzymes or chemical modifications. Therefore, the correct answer is A as ribosomes play a crucial role in protein synthesis.

Correct Answer: D

Rationale: The correct answer is D because an anticodon is a nucleotide triplet in a tRNA molecule that pairs with a complementary codon on mRNA during translation. Step 1: Anticodons are found in tRNA, not in DNA or mRNA. Step 2: Anticodons specifically bind to codons on mRNA to ensure correct amino acid incorporation into the growing polypeptide chain, making option D the most accurate choice. Option A is incorrect as replication does not involve anticodons. Option B is incorrect because codons, not anticodons, are found on mRNA molecules. Option C is incorrect as it describes a codon on DNA, not an anticodon on tRNA.