microbiology chapter 13 test bank

Correct Answer: B

Rationale: The correct answer is B: BCG. Bacille Calmette-Gu�rin (BCG) vaccine is used for the prevention of tuberculosis. BCG is a live attenuated strain of Mycobacterium bovis that helps the immune system recognize and fight off tuberculosis bacteria. Specific anti-tuberculosis serum (A) is not used for prevention but for treatment. DTP (C) stands for diphtheria, tetanus, and pertussis vaccine, not specific for tuberculosis prevention. PPD (D) is a tuberculin skin test used for diagnosis, not prevention. Thus, BCG is the correct choice for tuberculosis prevention.

Correct Answer: A

Rationale: The correct answer is A because immunofluorescence slides are examined under a fluorescence microscope with a x100 oil immersion objective. This is because immunofluorescence relies on the detection of fluorescently labeled antibodies, which emit light when excited by a specific wavelength. Using a fluorescence microscope allows for visualization of these labeled antibodies at high magnification and resolution, enabling precise examination of cellular structures and protein localization. Choice B is incorrect as light microscopes are not suitable for visualizing fluorescence. Choice C is incorrect as electron microscopes use electrons, not light, for imaging, which is not compatible with immunofluorescence. Choice D is incorrect as there is a specific method for examining immunofluorescence slides.

Correct Answer: A

Rationale: Step 1: Pear-shaped protozoa with two nuclei, four pairs of flagella, two axostyles, and a ventral adhesive disc match the characteristics of Giardia lamblia. Step 2: Giardia lamblia is commonly found in the duodenum and causes giardiasis. Step 3: Lamblia is the correct answer as it specifically matches all the described characteristics. Summary: B, Toxoplasma, is incorrect as it does not match the described features. C, Leishmania, does not have the specific characteristics mentioned. D, Intestinal trichomonad, does not match the detailed descriptions given.

Correct Answer: B

Rationale: The correct answer is B: Contains single-stranded RNA with negative polarity. Measles virus is an enveloped virus with a single-stranded RNA genome that is negative-sense. This means it must first be transcribed into positive-sense RNA before it can be translated. Choice A is incorrect as the measles virus does have an outer envelope. Choice C is incorrect as measles virus belongs to the family Paramyxoviridae, not Togaviridae. Choice D is incorrect as measles virus can be grown on cell cultures, specifically on Vero cells.

Correct Answer: B

Rationale: The correct answer is B: eukaryotic. Eukaryotic cells have a well-defined nucleus that encloses the genetic material. The nucleus is a distinguishing feature of eukaryotic cells, separating them from prokaryotic cells (choice A) which lack a nucleus. Anucleate cells (choice C) are cells that do not have a nucleus at all. Haploid cells (choice D) contain a single set of chromosomes, but this does not necessarily imply the presence of a nucleus. Therefore, the presence of a nucleus is what categorizes cells as eukaryotic.