microbiology a systems approach test bank

Correct Answer: B

Rationale: The correct answer is B: Gram's staining method. The rationale for this is as follows: Gentian violet and Lugol's solution are used as primary and mordant stains in the Gram's staining method. Spiritus 960 is used as a decolorizer, and water fuchsin is used as a counterstain. This staining method is commonly used in microbiology to differentiate bacteria into Gram-positive and Gram-negative based on their cell wall characteristics. A: Leffler's staining method is used for the identification of diphtheria bacilli. C: Ziehl-Neelsen's staining method is used for acid-fast bacteria like Mycobacterium tuberculosis. D: Neisser's staining method is used for the identification of Neisseria species. In summary, the use of specific reactives and stainers in the question indicates the application of the Gram's staining method for bacterial differentiation.

Correct Answer: A

Rationale: The correct answer is A: Amebiasis. Amebiasis is caused by the parasite Entamoeba histolytica, presenting with symptoms like acute abdominal pain, bloody diarrhea with mucus, and inconstant shape organisms seen in fecal smear. The presence of erythrocytes supports the diagnosis due to the parasite's ability to invade intestinal mucosa causing bleeding. Choice B (Lambliasis) presents with different symptoms like steatorrhea and fatigue. Choice C (Schistosomiasis) is mainly associated with liver and urinary symptoms. Choice D (Intestinal trichomoniasis) commonly presents with watery diarrhea and does not typically cause bloody stools. Therefore, based on the patient's symptoms and fecal smear findings, Amebiasis is the most likely diagnosis.

Correct Answer: C

Rationale: The correct answer is C because diphtheria is primarily spread through respiratory droplets and physical contact with an infected person. The bacteria responsible for diphtheria is transmitted through coughing, sneezing, or sharing personal items. Swimming in contaminated water (A) does not spread diphtheria. Transmission (B) is a broad term and does not specifically address the mode of transmission for diphtheria. Contaminated food (D) is not a typical route of diphtheria transmission. Therefore, option C is the most accurate choice based on the known mode of transmission for diphtheria.

Correct Answer: A

Rationale: Peptidoglycan is a major component of bacterial cell walls, providing structural support and protection. The correct answer is A because peptidoglycan is typically found in the cell wall of bacteria. The cell wall is crucial for maintaining the shape of the bacterial cell and protecting it from external stresses. The plasma membrane (choice B) is primarily composed of phospholipids and proteins, not peptidoglycan. The nucleus (choice C) is found in eukaryotic cells and contains genetic material, not peptidoglycan. The endoplasmic reticulum (choice D) is an organelle involved in protein synthesis and lipid metabolism, but it does not contain peptidoglycan. Therefore, the correct answer is A as peptidoglycan is typically found in the cell wall of bacteria.

Correct Answer: D

Rationale: The correct answer is D: All of the above. Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae are all bacteria associated with causing bacterial meningitis. Neisseria meningitidis commonly causes meningococcal meningitis, Haemophilus influenzae can cause H. influenzae type b meningitis, and Streptococcus pneumoniae is a common cause of pneumococcal meningitis. Therefore, all three bacteria are known to be causative agents of bacterial meningitis. The other choices are incorrect as they do not encompass all the bacteria associated with causing bacterial meningitis.