microbiology chapter 1 test bank

Correct Answer: D

Rationale: The correct answer is D because Borrelia burgdorferi cannot be detected with Giemsa stain. Giemsa stain is commonly used to visualize blood smears for malaria parasites but is not effective for Borrelia burgdorferi detection. A: Borrelia burgdorferi can be cultivated on blood agar - This is correct. Borrelia burgdorferi can be grown in culture on a special medium like Barbour-Stoenner-Kelly (BSK) agar. B: Borrelia burgdorferi is flexible, motile spirochete - This is correct. Borrelia burgdorferi is a spiral-shaped bacterium that moves using a corkscrew motion. C: Borrelia burgdorferi is transmitted by arthropods- ticks - This is correct. Borrelia burgdorferi is primarily transmitted to humans through the bite of infected ticks, particularly Ixodes

Correct Answer: A

Rationale: The correct answer is A because spores are heat-resistant and require high temperatures for destruction. Autoclaving at 121�C for 15-20 minutes ensures spore destruction due to the high pressure and temperature. Choice B (heating to 80�C) is insufficient to destroy spores. Choice C (boiling at 100�C for 10 minutes) is also inadequate as spores require higher temperatures for elimination. Choice D (None of them) is incorrect as autoclaving is the standard method for spore sterilization.

Correct Answer: A

Rationale: The correct answer is A: Yersinia pestis. Y. pestis causes bubonic plague, characterized by enlarged, painful lymph nodes called buboes. The Gram-negative rods with a safety pin appearance are seen on Gram stain of Y. pestis. The other choices are incorrect because Francisella tularensis causes tularemia, not lymphadenitis with safety pin appearance. Brucella abortus causes brucellosis, which typically does not present with lymphadenitis. Clostridium botulinum causes botulism, which is not associated with lymphadenitis or safety pin appearance.

Correct Answer: C

Rationale: Rationale: 1. Shigella invades and multiplies within the intestinal epithelial cells, causing inflammation and tissue damage locally. 2. It does not spread systemically, unlike Yersinia enterocolitica and Listeria monocytogenes. 3. Shigella's pathogenesis is focused on invasion and multiplication at the site of infection, leading to symptoms of dysentery. 4. Yersinia enterocolitica and Listeria monocytogenes can disseminate beyond the gastrointestinal tract, causing systemic infections. Summary: - A (Yersinia enterocolitica) and B (Listeria monocytogenes) are incorrect as they can generalize the infectious process. - C (Shigella) is correct as it mainly involves invasion and intraepithelial multiplication without spreading systemically.

Correct Answer: A

Rationale: Step-by-step rationale: 1. Mycobacterium tuberculosis is the causative agent of tuberculosis. 2. Mycobacterium tuberculosis is a slow-growing, acid-fast bacterium. 3. It primarily infects the lungs and can spread to other parts of the body. 4. Bacillus anthracis causes anthrax, Clostridium tetani causes tetanus, and Treponema pallidum causes syphilis. 5. Therefore, choice A, Mycobacterium tuberculosis, is the correct answer for the bacteria responsible for tuberculosis.