microbiology an introduction 12th edition test bank

Correct Answer: A

Rationale: The correct answer is A: Entamoeba histolytica. This is because it is a protozoan parasite known to cause amoebic dysentery, presenting with chronic diarrhea and cysts with four nuclei in the stool sample. Giardia lamblia (B) causes giardiasis with trophozoites and cysts with two nuclei. Balantidium coli (C) is a ciliated parasite causing dysentery with large trophozoites. Trichomonas hominis (D) is a non-pathogenic flagellate often found in the human intestine. Thus, based on the specific characteristics of the cysts and clinical presentation, Entamoeba histolytica is the most likely causative agent.

Correct Answer: A

Rationale: The correct answer is A: nematode. Trichinosis is caused by the roundworm Trichinella spiralis, a type of nematode. Nematodes are multicellular parasites commonly found in raw or undercooked meat. Viruses, bacteria, and fungi do not cause trichinosis. Viruses are not parasitic worms, bacteria are single-celled organisms, and fungi are eukaryotic organisms distinct from nematodes. Therefore, the ingestion of nematodes, not viruses, bacteria, or fungi, leads to trichinosis.

Correct Answer: A

Rationale: The correct answer is A: 96% ethanol. In Gram's staining method, after applying gentian violet, Lugol's solution, and aqueous fuchsin, the next step is to use a decolorizing agent like 96% ethanol to wash away the excess stain from the Gram-negative bacteria. This step is crucial as it helps differentiate between Gram-positive and Gram-negative bacteria based on their cell wall properties. The other choices are incorrect because sulfuric acid is not used in Gram's staining method, methylene blue is typically used in other staining techniques like the simple stain, and carbolic fuchsin is not a standard reagent in the Gram's staining process.

Correct Answer: C

Rationale: The correct answer is C: RNA-dependent RNA polymerase. This enzyme is required for replicating viral RNA due to its ability to catalyze the synthesis of RNA using an RNA template. Reverse transcriptase (A) is used to transcribe RNA into DNA, not replicate viral RNA. DNA polymerase (B) is used for replicating DNA, not RNA. Ligase (D) is involved in joining DNA fragments, not in replicating viral RNA. Therefore, RNA-dependent RNA polymerase is the only enzyme that specifically performs the function required for replicating viral RNA.

Correct Answer: B

Rationale: The correct answer is B: prophage. In the repressed stage, a phage integrates its DNA into the host cell's chromosome, becoming a prophage. This integration allows the phage to replicate along with the host cell without causing immediate lysis. A: Antiphage is not a term used to describe a phage in a repressed stage. C: Virulent phage refers to a phage that immediately causes lysis of the host cell. D: Latent phage is not a commonly used term in this context.