Microbiology Basic and Clinical Principles Test Bank

Correct Answer: B

Rationale: The correct answer is B: Precipitins. When a granular sediment forms at the bottom of the test-tubes, it indicates the formation of antigen-antibody complexes that are insoluble and precipitate out of the solution. Precipitins are antibodies that specifically react with antigens to form insoluble complexes. Agglutinins cause clumping of cells, opsonins enhance phagocytosis, and bacteriolysins lyse bacteria. These processes do not result in the formation of a granular sediment, making them incorrect choices in this context.

Correct Answer: A

Rationale: The correct answer is A: Delayed hypersensitivity reaction. In granuloma development in tuberculosis, macrophages present antigen to T cells, leading to a delayed hypersensitivity reaction. This triggers the recruitment of more macrophages and formation of granulomas. Antibody-dependent cytotoxicity (B) and complement-dependent cytotoxicity (C) involve antibodies and complement proteins in immediate hypersensitivity reactions, which are not the primary mechanisms in tuberculosis. Anaphylactic reaction (D) is an acute systemic allergic reaction mediated by IgE antibodies, not relevant to granuloma formation in tuberculosis.

Correct Answer: A

Rationale: The correct answer is A: Vibrios. Vibrios are gram-negative curved bacteria that are motile and do not form spores or capsules. The description of the bacteria in the question fits the characteristics of Vibrios. Vibrios are commonly found in aquatic environments and some species can cause gastrointestinal infections in humans. B: Spirochetes are also gram-negative curved bacteria, but they are spiral-shaped and have unique axial filaments for motility. The description in the question does not match the characteristics of spirochetes. C: Clostridia are gram-positive, spore-forming bacteria. The question specifically states that the bacteria are gram-negative and do not form spores, ruling out Clostridia. D: Corynebacteria are gram-positive, non-spore-forming bacteria. The description in the question does not match the characteristics of Corynebacteria.

Correct Answer: A

Rationale: The correct answer is A because Gram-negative bacteria have a unique cell envelope structure consisting of a cytoplasmic membrane, a peptidoglycan layer, and an outer membrane. The cytoplasmic membrane is the innermost layer that separates the cytoplasm from the external environment. The peptidoglycan layer provides structural support to the cell. The outer membrane acts as an additional protective barrier and contains lipopolysaccharides. Choice B is incorrect because Gram-negative bacteria have an outer membrane in addition to the cytoplasmic membrane and peptidoglycan layer. Choice C is incorrect because Gram-negative bacteria have more than just a peptidoglycan layer in their cell envelope. Choice D is incorrect as the cell envelope of Gram-negative bacteria does have specific components.

Correct Answer: B

Rationale: Step-by-step rationale for why choice B (Tuberculin is introduced) is correct: 1. Tuberculin test helps identify individuals with latent TB infection. 2. Negative skin-allergic samples suggest absence of active TB. 3. Introducing tuberculin can help confirm latent TB infection. 4. Antibiotics are not prescribed for negative skin-allergic samples. 5. Vaccines are not administered for TB diagnosis. Summary: - Choice A is incorrect as antibiotics are not prescribed for negative skin-allergic samples. - Choice C is incorrect as vaccines are not used for TB diagnosis. - Choice D is incorrect as introducing tuberculin is the appropriate step in this scenario.