ATI RN
Mastering Microbiology Test Bank Questions
Question 1 of 5
A centrifugate of urine sample obtained from patient with suspected renal tuberculosis was used to make a slide mount for microscopy. What method should be used to stain the slide and detect the causative agent?
Correct Answer: A
Rationale: The correct answer is A: Zielh-Neelsen stain. This stain is specifically used to detect acid-fast bacteria like Mycobacterium tuberculosis, the causative agent of renal tuberculosis. The staining process involves using carbol fuchsin to penetrate the mycolic acid layer of the bacterial cell wall, making it resistant to decolorization with acid-alcohol. This allows the red stained bacteria to be visualized under a microscope. Loeffler stain (B) is used for staining bacteria like diphtheria bacilli, Gram stain (C) is used for bacterial classification based on cell wall properties, and Aujeszky stain (D) is not a recognized staining method for detecting acid-fast bacteria.
Question 2 of 5
Precipitation reaction can be converted into agglutination reaction by coating soluble antigen onto
Correct Answer: D
Rationale: The correct answer is D: All of these. Coating soluble antigen onto Bentonite particles, RBCs, or Latex particles can convert a precipitation reaction into an agglutination reaction. This is because soluble antigens bind to their specific antibodies, causing the particles to clump together, visible as agglutination. Bentonite particles, RBCs, and Latex particles all provide surfaces for the antigen-antibody interaction to occur, leading to agglutination. Therefore, all of these options are correct for converting precipitation reactions to agglutination reactions.
Question 3 of 5
Which of the following pathogens has the widest infectious spectrum
Correct Answer: A
Rationale: The correct answer is A, Anthrax bacillus, because it has the widest infectious spectrum among the options provided. Anthrax bacillus can infect a wide range of hosts, including humans and various animals. It is known for its ability to survive in different environments and cause disease through various routes of exposure. In contrast, choices B, C, and D have more limited infectious spectra. Mumps virus primarily infects humans, the causative agent of gonorrhea mainly affects humans through sexual contact, and the rabies virus typically infects mammals through bites from infected animals. Therefore, Anthrax bacillus is the correct choice due to its broader range of hosts and modes of transmission compared to the other options.
Question 4 of 5
A patient has a suspected pneumonia. In his sputum there were revealed gram-positive diplococci, prolonged with the slightly pointed opposite ends. What microorganisms are revealed in the sputum?
Correct Answer: A
Rationale: Rationale: The description matches that of Streptococcus pneumoniae, which appears as gram-positive diplococci with slightly pointed opposite ends. Staphylococcus aureus, Neisseria gonorrhoeae, and Neisseria meningitidis do not match the specific characteristics described in the question. Staphylococcus aureus appears in grape-like clusters, while Neisseria species are typically seen as diplococci but lack the pointed shape described. Therefore, the correct answer is A: Staphylococcus aureus.
Question 5 of 5
Which of the following is characteristic of obligate anaerobes?
Correct Answer: C
Rationale: The correct answer is C: Obligate anaerobes cannot tolerate oxygen. They lack the enzymes needed to detoxify reactive oxygen species generated in the presence of oxygen. This makes them unable to survive in oxygen-rich environments. Choice A is incorrect because obligate anaerobes do not require oxygen to survive. Choice B is incorrect because obligate anaerobes specifically require the absence of oxygen. Choice D is incorrect because obligate anaerobes cannot even tolerate low levels of oxygen.
Similar Questions
Join Our Community Today!
Join Over 10,000+ nursing students using Nurselytic. Access Comprehensive study Guides curriculum for ATI-RN and 3000+ practice questions to help you pass your ATI-RN exam.
Subscribe for Unlimited Access