ATI RN
Mastering Microbiology Test Bank Questions
Question 1 of 5
A centrifugate of urine sample obtained from patient with suspected renal tuberculosis was used to make a slide mount for microscopy. What method should be used to stain the slide and detect the causative agent?
Correct Answer: A
Rationale: The correct answer is A: Zielh-Neelsen stain. This stain is specifically used to detect acid-fast bacteria like Mycobacterium tuberculosis, the causative agent of renal tuberculosis. The staining process involves using carbol fuchsin to penetrate the mycolic acid layer of the bacterial cell wall, making it resistant to decolorization with acid-alcohol. This allows the red stained bacteria to be visualized under a microscope. Loeffler stain (B) is used for staining bacteria like diphtheria bacilli, Gram stain (C) is used for bacterial classification based on cell wall properties, and Aujeszky stain (D) is not a recognized staining method for detecting acid-fast bacteria.
Question 2 of 5
By precipitation of tuberculin, a purified protein derivative (PPD) is obtained. It is used in:
Correct Answer: A
Rationale: The correct answer is A: Mantoux test. The Mantoux test is used to screen for tuberculosis by injecting PPD intradermally. The presence of a delayed hypersensitivity reaction indicates prior exposure to Mycobacterium tuberculosis. The Schick test (B) is used to test for immunity to diphtheria, while the Dick test (C) is used to test for immunity to scarlet fever. Therefore, the correct use of PPD is in the Mantoux test for tuberculosis screening.
Question 3 of 5
Which bacterial structure helps in the movement towards or away from chemical stimuli?
Correct Answer: A
Rationale: Flagella are the correct answer because they are long, whip-like structures that aid in bacterial movement towards or away from chemical stimuli. They rotate like propellers, allowing the bacterium to navigate its environment. Pili are used for attachment, not movement. Capsules are involved in protection and adherence, not chemotaxis. Fimbriae help in attachment to surfaces, not in movement towards stimuli.
Question 4 of 5
The key function of bacterial capsules is:
Correct Answer: B
Rationale: The correct answer is B: resisting phagocytosis. Bacterial capsules are composed of polysaccharides that help bacteria evade phagocytosis by immune cells. This physical barrier prevents immune cells from engulfing and destroying the bacteria. Facilitating motility (A), synthesizing DNA (C), and producing energy (D) are not primary functions of bacterial capsules. Motility is usually facilitated by flagella, DNA synthesis occurs in the bacterial chromosome, and energy production is typically carried out by metabolic processes within the bacterial cell.
Question 5 of 5
An inflammatory disease that can develop as a complication following a group A streptococcal infection is:
Correct Answer: B
Rationale: The correct answer is B: rheumatic fever. Rheumatic fever is an inflammatory disease that can occur as a complication following a group A streptococcal infection. This is known as a post-streptococcal complication. It typically affects the heart, joints, skin, and brain. Endocarditis (choice A) is also a complication of streptococcal infections, but it is an infection of the heart valves, not an inflammatory disease like rheumatic fever. Nephritis (choice C) is inflammation of the kidneys and is not directly related to streptococcal infections. Cytomegalovirus (choice D) is a different viral infection and is not associated with streptococcal infections.
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