microbiology chapter 11 test bank

Correct Answer: A

Rationale: Rationale: 1. Spore-forming, Gram-positive rods: Characteristics shared by Clostridium and Bacillus. 2. Motile and gas production in nutrient broth: Clostridium perfringens is motile and produces gas, distinguishing it from Bacillus species. 3. Clostridium perfringens causes gas gangrene, correlating with gas production. 4. Bacillus anthracis causes anthrax, not related to wound infections. 5. Clostridium tetani causes tetanus, not associated with gas production. 6. Bacillus cereus causes food poisoning, not commonly associated with wound infections. Summary: - A is correct (Clostridium perfringens) due to motility, gas production, and wound infection correlation. - B (Bacillus anthracis) causes anthrax, not wound infections. - C (Clostridium tetani) causes tetanus, not gas production. - D (B

Correct Answer: D

Rationale: The correct answer is D: Proteus mirabilis. The patient's symptoms of lower abdominal pain, back pain, frequent/painful urination, and the presence of gram-negative oxidase-positive bacilli forming green mucoid colonies with a specific smell suggest a urinary tract infection caused by Proteus mirabilis. This bacterium is known for causing UTIs and is characterized by its swarming motility and distinctive odor. A: Pseudomonas aeruginosa is commonly associated with hospital-acquired infections but does not typically cause UTIs with the specific characteristics described. B: Mycoplasma pneumonia is a bacterium that causes respiratory infections, not UTIs. C: Str.pyogenes (Streptococcus pyogenes) is a common cause of strep throat and skin infections, not UTIs. D: Helicobacter pylori is associated with gastritis and peptic ulcers, not UTIs.

Correct Answer: A

Rationale: The correct answer is A: Β-lactamase. In ELISA, enzymes like alkaline phosphatase, horseradish peroxidase, and Β-galactosidase are commonly used as labels to detect the presence of specific molecules. Β-lactamase is not typically used in ELISA as it is an enzyme used to hydrolyze β-lactam antibiotics. Therefore, it does not serve the purpose of detecting specific molecules in ELISA. Alkaline phosphatase, horseradish peroxidase, and Β-galactosidase are preferred enzymes in ELISA due to their ability to produce a detectable signal when bound to specific molecules.

Correct Answer: B

Rationale: The correct answer is B: phase-contrast microscope. This type of microscope is ideal for observing living microorganisms because it enhances the contrast of transparent specimens without the need for staining or fixing, allowing for clear visualization of internal structures and movements. Bright-field microscopes (A) are suitable for observing fixed and stained specimens, not living organisms. Fluorescence microscopes (C) are used to detect specific cellular components tagged with fluorescent markers, not ideal for observing overall morphology. Electron microscopes (D) use electron beams and are best for viewing detailed structures at high magnification, but are not suitable for observing living organisms due to the need for vacuum conditions.

Correct Answer: D

Rationale: The correct answer is D: Middlebrook's medium. This medium is specifically designed for the growth of Mycobacterium tuberculosis due to its low concentration of malachite green, which inhibits the growth of other bacteria but not Mycobacterium species. Middlebrook's medium also contains various nutrients that support the growth of Mycobacterium tuberculosis. A: Middlebrook's medium is the correct answer, not incorrect. B: Heart infusion broth is a general-purpose medium, not specific for Mycobacterium tuberculosis. C: MacConkey agar is selective for gram-negative bacteria and would not support the growth of Mycobacterium tuberculosis. In summary, Middlebrook's medium is the ideal choice for cultivating Mycobacterium tuberculosis due to its specific formulation that promotes the growth of this pathogen while inhibiting other bacteria.